Proteome adaptations under contrasting soil phosphate regimes of Rhizophagus irregularis engaged in a common mycorrhizal network

For many plants, their symbiosis with arbuscular mycorrhizal fungi plays a key role in the acquisition of mineral nutrients such as inorganic phosphate (Pi), exchange for assimilated carbon. To study gene regulation and function symbiotic partners, we others have used compartmented microcosms which extra-radical mycelium (ERM), responsible nutrient supply was separated by fine nylon nets from associated host roots could be harvested analysed isolation. Here, model system to perform quantitative comparative protein profiling ERM Rhizophagus irregularis BEG75, forming common network (CMN) between poplar sorghum under long-term high- or low-Pi fertilization regime. Proteins were extracted liquid chromatography-tandem mass spectrometry. This workflow identified total 1301 proteins, among 162 displayed differential amount during Pi limitation, monitored spectral counting. Higher abundances recorded proteins involved mobilization external Pi, secreted acid phosphatase, 3?,5?-bisphosphate nucleotidase, calcium-dependent phosphotriesterase. also case intracellular phospholipase lysophospholipases that are initial degradation phospholipids membrane lipids mobilize internal Pi. In Pi-deficient conditions. The CMN proteome especially enriched assigned beta-oxidation, glyoxylate shunt gluconeogenesis, indicating storage rather than carbohydrates fuelled carbon source support hyphal growth energy requirements. contrasting pattern expression AM-specific fatty biosynthetic genes two plants suggests low conditions, provision fungal is mediated but not poplar. Loss enzymes arginine synthesis coupled breakdown nitrogen sources intercellular purines amino acids, view ammonium through pathway may reduced proteomic highlights functioning limiting provides new perspectives plant fungi.